emboss的linux版本的源代码
源代码在线查看: eprimer3.output
Output files for usage example File: hsfau.eprimer3 # EPRIMER3 RESULTS FOR HSFAU1 # FORWARD PRIMER STATISTICS: # considered 16422 # GC content failed 156 # low tm 3616 # high tm 9306 # high any compl 9 # high end compl 26 # long poly-x seq 45 #high 3' stability 304 # ok 2960 # REVERSE PRIMER STATISTICS: # considered 16330 # GC content failed 161 # low tm 3580 # high tm 9309 # high any compl 2 # high end compl 9 # long poly-x seq 66 #high 3' stability 301 # ok 2902 # PRIMER PAIR STATISTICS: # considered 2652 # unacceptable product size 2444 # high any compl 2 # high end compl 20 # ok 186 # Start Len Tm GC% Sequence 1 PRODUCT SIZE: 202 FORWARD PRIMER 801 20 59.72 60.00 CCCAGGAGCTACACACCTTC REVERSE PRIMER 983 20 59.81 55.00 GGAGCACGACTTGATCTTCC 2 PRODUCT SIZE: 203 FORWARD PRIMER 1810 20 59.43 55.00 GAAGAAGACAGGTCGGGCTA REVERSE PRIMER 1993 20 59.99 60.00 GAAGCACACCTCTCCCTGAG 3 PRODUCT SIZE: 204 FORWARD PRIMER 1810 20 59.43 55.00 GAAGAAGACAGGTCGGGCTA REVERSE PRIMER 1994 20 59.99 55.00 AGAAGCACACCTCTCCCTGA 4 PRODUCT SIZE: 196 FORWARD PRIMER 1266 20 59.43 60.00 GGGAGACTGAGCCAGAGTGT REVERSE PRIMER 1442 20 60.06 50.00 GCGCCCAATAGGTAATGAGA 5 PRODUCT SIZE: 205 FORWARD PRIMER 801 20 59.72 60.00 CCCAGGAGCTACACACCTTC REVERSE PRIMER 986 20 60.41 55.00 CCAGGAGCACGACTTGATCT